Abstract
Establishment of efficient protocol for high-frequency of indirect regeneration of plantlets has a vital role in the analysis of genetic material in mass propagated plants. The optimal levels of growth regulators and light conditions were investigated on callus induction and organogenesis of cultured grapevine from in vitro tissues. Maximum calluses and shoots were produced by using medium supplemented with four different growth regulators as alone or in combinations. An observation of maximum calluses (51%) were recorded when 0.5 mg/l thidiazuron (TDZ), was combined with 0.5 mg/l indole-3-butyric acid (IBA) for Chenin blanc cv. Produced calluses were observed with different size and nearly similar colors. In this experiment, shoot initiation was observed in dark condition. The light condition did not induce the shoot on the same dark treatment. Different concentrations of 6-benzylaminopurine (BAP) and TDZ tested alone were not induced at any shoots from callus, but re-callusing the explant. Thus, shoot induction was observed when different concentration of BAP and TDZ were combined with auxins. The calluses produced from leaf did not produce high percentages of shoots. Further studies are needed to optimize the maximum percentage of somatic embryogenesis. Key words: Callus, growth regulators, organogenesis, grapevine, dark.
Highlights
The organogenesis is a biotechnological tool used for obtaining mass production of mother plant with high quality of health (Bettoni et al, 2015)
Upper most leaf explant induced from the induction medium was wounded and cultured in the medium supplemented with four different growth regulators (BAP, indole-3-butyric acid (IBA), TDZ and NAA) as alone or in combinations, to produce calluses
After culturing leaf explants in different concentrations of growth regulators, different amount of calluses were observed at dark
Summary
The organogenesis is a biotechnological tool used for obtaining mass production of mother plant with high quality of health (Bettoni et al, 2015). The explants can be grown into whole plant or produce callus. The produced callus can be utilized to regenerate plantlets or to extract or manipulate some primary and secondary metabolites (Pande and Gupta, 2013). Plant mass production can be affected by several factors such as light, temperature, plant varieties, and type of explant, components of media, sources and orientation of explants (Kumar and Raddy, 2011). The most common culture temperature range has been between 20 and 27°C, but optimal temperatures vary widely, depending on genotype (Kumar and Raddy, 2011)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
