Abstract
In the present study, we investigated the effect of the aqueous extract of Mucuna pruriens, against cisplatin induced oxidative stress and nephrotoxicity in rats. Nephrotoxicity was induced by a single dose of cisplatin (5 mg/kg body weight i.p.). Cisplatin administration resulted in significant increases in urine volume, serum creatinine and urea and significant decrease in creatinine clearance and urinary sodium in comparison with control. Also, the renal tissue from the cisplatin treated rats showed significant decreases in the kidney glutathione content, superoxide dismutase and catalase activity and a significant increase in lipid peroxides levels. Seven days after M. pruriens extract at a dose of 200 and 400 mg/kg plus cisplatin treatments significantly decrease urea, creatinine and significantly increase creatinine clearance levels as compared to cisplatin rats in a dose dependent manner. In addition, M. pruriens prevented the rise of lipid peroxides and the reduction of superoxide dismutase, catalase and glutathione activities in a dose dependent manner. These results suggest that M. pruriens extract has protective effects against cisplatin induced oxidative stress and nephrotoxicity in rats. Key words: Mucuna pruriens, cisplatin, lipid peroxidation, free radicals.
Highlights
Cisplatin, one of the most potent and widely used anticancer drugs containing platinum, is highly effective against many tumors, including testicular, small cell lung, head and neck, and bladder carcinomas (Meyer et al, 1994)
The xenobiotic-induced alterations in kidney functions are characterized by signs of injury, such as changes in urine volume, creatinine clearance, in glutathione (GSH) status, increase of lipid peroxidation (LPO)
The seeds were powdered in a mechanical grinder. 1 kg seed powder of M. pruriens was initially defatted with 750 ml of petroleum ether (60-80°C) aqueous extract was prepared by cold maceration method in that extract was shaken intermediately and CHCl3 was added to prevent bacterial growth
Summary
Cisplatin (cis-diamminedichloroplatinum II, CP), one of the most potent and widely used anticancer drugs containing platinum, is highly effective against many tumors, including testicular, small cell lung, head and neck, and bladder carcinomas (Meyer et al, 1994). CP induced nephrotoxicity is closely associated with an increase in LPO in the kidney tissues. This antitumoural drug causes generation of reactive oxygen species (ROS), such as superoxide anion and hydroxyl radical, to deplete of GSH levels and to inhibit the activity of antioxidant enzymes in renal tissue. ROS may produce cellular injury and necrosis via several mechanisms including peroxidation of membrane lipids, protein denaturation and DNA damage (Kim et al, 1997; Mora et al, 2003). M. pruriens seed extract has been reported to attenuate progression of renal damage in streptozotocin-induced diabetic mice (Grover et al, 2001). In light of above objective, current investigation was to study effect of M. pruriens seed extract in cisplatin induced nephrotoxicity and oxidative stress damage in rats
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