Abstract
The genetic improvement of production traits can be developed through marker assisted selection. Insulin-like growth factor I (IGF-I) is a member of the somatotrophic axis which has a remarkable variation of its biological effect including protein synthesis and skeletal growth. This study aimed to detect the genetic polymorphism of IGF-1 in different Egyptian sheep and goat breeds. The amplified fragments at 320-bp were digested with HaeIII endonuclease and the results show the presence of three different genotypes: CC (15.71%), CG (29.29%) and GG (55.0%). The nucleotide sequence analysis of C and G alleles declared the presence of a single nucleotide polymorphism (C→G) at position 282. The nucleotide sequences of alleles C and G in sheep and goat were submitted to GenBank with the accession number: KX432965, KX432966, KX432967 and KX432968, respectively. In conclusion, a nucleotide substitution (C→G) was detected in IGF-I gene in Egyptian sheep and goat breeds resulting in the presence of three different genotypes; CC, CG and GG. The association of IGF-I polymorphism with different growth trait parameters were reported at significant levels, so, the genetic and SNP variations in IGF-I gene may be a potential molecular marker for growth traits in different Egyptian sheep and goat breeds. Key words: IGF-1, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), DNA sequencing, sheep, goat.
Highlights
Molecular genetics techniques are of great interest in the identification of genetic variations in genetic markers which are associated with different production and reproduction traits in farm animals (Jiang et al, 2002; Arora and Bhatia, 2006; Missohou et al, 2006)
There are number of single genes associated with mammary or muscle growth, development and function which were studied as excellent candidates for linkage relationships with quantitative traits of economic importance
One of the most important member of the somatotrophic axis is insulin-like growth factor I (IGF-I) which has a remarkable variation of its biological effect like protein synthesis and skeletal growth (Froesch et al, 1985; Baxter, 1985; Clemmons et al, 1987)
Summary
Molecular genetics techniques are of great interest in the identification of genetic variations in genetic markers which are associated with different production and reproduction traits in farm animals (Jiang et al, 2002; Arora and Bhatia, 2006; Missohou et al, 2006). These genetic variations affect the physiological pathways that lead to quantitative variations in different phenotype characteristics (Schwerine et al, 1995; Lan et al, 2007). One of the most important member of the somatotrophic axis is insulin-like growth factor I (IGF-I) which has a remarkable variation of its biological effect like protein synthesis and skeletal growth (Froesch et al, 1985; Baxter, 1985; Clemmons et al, 1987)
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