Abstract
The growth kinetics and modeling of ethanol production from inulin by Pichia caribbica (KC977491) were studied in a batch system. Unstructured models were proposed using the logistic equation for growth, the Luedeking-Piret equation for ethanol production and modified Leudeking-Piret model for substrate consumption. Kinetic parameters (X0, μm, m, n, p and q) were determined by nonlinear regression, using Levenberg-Marquart method implemented in a Mathcad program. Since the production of ethanol was associated with P. caribbica cell growth, a good agreement between model predictions and experimental data was obtained. Indeed, significant R2 values of 0.91, 0.96, and 0.95 were observed for biomass, ethanol production and substrate consumption, respectively. Furthermore, analysis of variance (ANOVA) was also used to validate the proposed models. According to the obtained results, the predicted kinetic values and experimental data agreed well . Finally, it is possible to predict the development of P. caribbica using these models. Key words : Pichia caribbica, inulin, bioethanol, numerical simulation.
Highlights
Bio-ethanol being a clean, safe and renewable resource has been considered as a potential alternative to the ever-decreasing fossil fuels (Martin et al, 2002; Wyman, 1994)
The supernatant w ere cleaned by cellulose acetate membrane (0.2 μm, Minisart Sartorius), the fructose consumption and ethanol production w ere investigated by HPLC under subsequent conditions follow ing the CWBI protocol: Agilent 1110 series (HP Chemstation softw are) w ith a Supelcogel C-610H column preceded by a Supelguard H pre-column . 0.1% H3 PO4 solution w as used as the isocratic mobile phase at a flow rate of 0.5 ml min-1 and a differential refractive index detector ( RID) w as heated at 35°C
The results show a good agreement with experimental data (R2 = 0.99), the logistic equation was found to be an appropriate kinetic model for successfully describing yeast cell growt h in batch fermentation of raw juice system
Summary
Bio-ethanol being a clean, safe and renewable resource has been considered as a potential alternative to the ever-decreasing fossil fuels (Martin et al, 2002; Wyman, 1994). Most of the industrial processes are currently based on hexose carbohydrates from starch or sucrose-containing biomass (Kumari and Pramanik, 2012; Duhan et al, 2013) Among these substrates, inulin has rec eived a major interest since it is present as a carbohydrate reserve in a large variety of plant roots and tubers such. Theoretical models have been proposed and used for the elucidation of metabolic steps and for the calculation of kinetic parameters (Ghos h et al, 2012). To our knowledge, this is the first report to study Pichia caribbica (KC977491) growth kinetics and the modeling of ethanol production from inulin by this yeast strain. The main objectives were to: (I) P roduce biomass and ethanol by P. caribbica (KC977491) in a batch system; (II) Propose unstructured models for growth and ethanol production t o predict a process of fermentation by P. caribbica (KC977491); (III) Validate the obtained results between the theoretical unstructured models and experimental data
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