English

Abstract

BACKGROUND Streptococcus pyogenes can affect most often in children with peak age related incidence between 11 to 15 years of age and all races of the world. ASO estimation as an early diagnosis can help to start early & accurate treatment. The objectives of this study were to determine the ASO levels among paediatric age group patients and to estimate the age and sex relation to ASO levels. METHODS A total of 200 patient samples were considered under study population for investigating Anti streptolysin O titres. All the serum samples were processed using Tulip Diagnostics ASO Kits. All blood samples of study population were collected and processed immediately by centrifugation at 3000 rpm for 15 minutes. Serum sample is extracted and processed by semi quantitative method for detection of Anti Streptolysin – O. RESULTS Majority of the patients were observed in the age group of 11 - 15 years i.e., 104 out of 200 (52 %) followed by 5-10 years makes up to 41 %. Male preponderance was noted which accounts for 56.5 % of total cases. Out of 68 male positive patients, 21 (30.8 %) had ASO titres of 200-400 IU / mL, 27 (39.7 %) had ASO titres of 400-800 IU / mL and 20 (29.4 %) had ASO titres of > 800 IU / mL. Out of 31 female ASO positive patients, 5 had (19.2 %) of ASO 200 - 400 IU / mL, 17 had (38.6 %) of ASO 400-800 IU/mL and 9 had (31.03 %) ASO titres of > 8 00 IU / mL. CONCLUSIONS Increase in ASO titres indicate recent infection, which can be tested easily and timely report. Many hospitals are choosing ASO test as a priority investigation to diagnose GAS infections. KEYWORDS Anti-Streptolysin O, Children