Abstract
Bletilla is a temperate, terrestrial genus of orchids containing 6 species. For the species whose whole genome is unknown, we used magnetic bead hybridization method to develop microsatellite Simple Repeat Polymorphoresis (SSR) for Bletilla striata and 9 primer sets were characterized in two wild populations of B. striata and one wild population of Bletilla ochracea. The number of alleles per locus ranged from 1 to 12. The expected and observed heterozygosities ranged from 0 to 0.7646 and 0 to 0.950 in B. striata, respectively. In B. ochracea, the expected and observed heterozygosities ranged from 0.296 to 0.871 and 0.05 to 1, respectively. The 9 pairs of primers we designed can be used to distinguish different ecotypes and species, and might be used for other subspecies or species in genera Bletilla. Key words: Bletilla striata, cross-species amplification, simple repeat polymorphoresis (SSR).
Highlights
The genus Bletilla, composting about six species, is endemic to Asia with a distribution pattern from N Myanmar and Indochina through China to Japan
For the species whose whole genome is unknown, we used magnetic bead hybridization method to develop microsatellite Simple Repeat Polymorphoresis (SSR) for Bletilla striata and 9 primer sets were characterized in two wild populations of B. striata and one wild population of Bletilla ochracea
Microsatellite markers are based on the amplification of internal sequences between microsatellites [intersimple sequence repeat (ISSR) markers] using PCR and looking for microsatellite loci contained within these sequences, taking into account that microsatellites are generally clustered within the plant genome, and microsatellite markers have been proven useful in assessing genetic diversity of populations in different species (López-Roberts et al, 2012; Caitlin et al, 2013)
Summary
The genus Bletilla, composting about six species, is endemic to Asia with a distribution pattern from N Myanmar and Indochina through China to Japan. F., includes art (Chinese painting and writing), the production of porcelain, and medicine (Lawler, 1984), as well as vegetable dyes for dyeing cloth in some minority people in Guizhou and Yunnan Province, China (Luo, person observation). Those species are commonly used as a horticultural subject covering both indoors and out cultivation in North American and European areas (Pridgeon, 2005). We describe microsatellite loci which developed from B. striata and test the transferability of those markers to other related species These loci were useful for further breeding superior cultivars and studies of genetic diversity, and contribute to knowledge on conservation of genus Bletilla
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