Abstract
Cucumber mosaic virus Ca-P1 strain, a P0 resistance-breaking virus, was isolated from the leaves of virus-infected Capsicum annuum ‘Manidda’ (a P0 resistant cultivar) in South Korea. Since CMV-Ca-P1 was first reported in 2006, this virus causing damage on pepper production has been constantly detected in South Korea. We constructed three CMV RNAi vectors based on the post-transcriptional gene silencing of defense system against virus infection. These independent vectors (hpCMV1, hpCMV2, and hpCMV2 + 1 RNAi vectors) were designed to produce dsRNAs containing each hpCMV1 (1270–1629 nt), hpCMV2 (1–300 nt), and hpCMV2 + 1 (fused hpCMV2 and hpCMV1) fragments, in RNA-1 (replicase gene) of CMV-Ca-P1, which were then confirmed by Agrobacterium-mediated transformation transient assay. Among these, dsRNAs expressed from the hpCMV2 + 1 vector showed resistance to both CMV-Ca-P1 and CMV-Fny (ordinary strain). To obtain high level of resistance to both CMV-Ca-P1 and CMV-Fny, transgenic Nicotiana benthamiana plants containing hpCMV2 + 1 vector were developed and conferred resistance to both CMV-Ca-P1 and CMV-Fny. This study contributes to the effective selection of target sequences that may inhibit CMV infection.
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