Engineering pH responsive carboxyethyl chitosan and oxidized pectin -based hydrogels with self-healing, biodegradable and antibacterial properties for wound healing

Abstract

As an important organ of the human body, effective protection of the skin during trauma is crucial. An ideal wound dressing should have adhesion, adsorption of wound secretions, and good antibacterial properties. Two kinds of natural polysaccharide-based hydrogels, carboxyethyl chitosan/oxidized pectin hydrogel (CEC/OP) and carboxyethyl chitosan/oxidized pectin/polyethyleneimine hydrogel (CEC/OP/PEI), were reported by using carboxyethyl chitosan as the matrix, and oxidized pectin and branched polyethyleneimine as the crosslinking agents. Both hydrogels could be formed in a short time and exhibited the pH responsively due to the presence of imine bond. Compared with carboxyethyl chitosan/oxidized pectin hydrogel, polyethyleneimine containing hydrogel can form gel quickly, a more compact and stable three-dimensional space network structure was formed, which exhibited better swelling performance, the swelling ration, rheology property, self-repair ability, and antibacterial performance. When the mass fractions of carboxyethyl chitosan and oxidized pectin solutions are 4 wt% and 9 wt%, respectively, the hydrogel exhibited an antibacterial efficiency of >96 % against both Staphylococcus aureus and Escherichia coli. After adding 0.75 wt% polyethyleneimine, the antibacterial efficiency of hydrogel could reach up to >98 %. More importantly, the polyethyleneimine containing hydrogel has a significant effect in the treatment of bacterially infected wounds.