Abstract
The engineering of renewable and sustainable protein-based light-to-energy converting systems is an emerging field of research. Here, we report on the development of supramolecular light-harvesting electrodes, consisting of the redox protein cytochrome c working as a molecular scaffold as well as a conductive wiring network and photosystem I as a photo-functional matrix element. Both proteins form complexes in solution, which in turn can be adsorbed on thiol-modified gold electrodes through a self-assembly mechanism. To overcome the limited stability of self-grown assemblies, DNA, a natural polyelectrolyte, is used as a further building block for the construction of a photo-active 3D architecture. DNA acts as a structural matrix element holding larger protein amounts and thus remarkably improving the maximum photocurrent and electrode stability. On investigating the photophysical properties, this system demonstrates that effective electron pathways have been created.
Highlights
Photosystem I (PSI) aus dem thermophilen Cyanobakterium Thermosynechococcus elongatus ist ein transmembraner Protein-Pigment-Superkomplex der photosynthetischen Elektronentransportkette
The crystal structure was solved to a resolution of 3,4 ̊A, no electron density corresponding to Cytochrom c aus Pferdeherz (Cyt cHH) was found
To verify the PSICyt c6 complex, the two proteins were crosslinked and the structure was solved by cryo electron microscopy to a resolution of 2,9 ̊A
Summary
In der Schule lernen wir, dass die Luft, die wir atmen, aus 78 % Stickstoff, 21 % Sauerstoff, 1 % Argon und 0,04 % Kohlenstoffdioxid besteht. Mit Erscheinen der Cyanobakterien stieg die Sauerstoffkonzentration drastisch an und das Leben, wie wir es heute kennen, konnte entstehen. Heute sind Cyanobakterien eine große Gruppe mit 300 Gattungen und mehr als 4000 isolierten Stammen (Shestakov und Karbysheva 2017; Maidak et al 2001). PCC 6803, welches sich durch eine naturliche Kompetenz fur gentechnische Modifikationen eignet, und die Gattungen Prochlorococcus und Synechococcus, welche mit 1026 bis 1027 Zellen die bedeutsamsten Primarproduzenten der Erde sind (Flombaum et al 2013). Entgegen seinem Gattungsnamen handelt es sich hierbei nicht um eine KTwo.obkeekloien,dgesarotnuHsdienirnwnediusemrauLfeitisenerianStetuarTbahclhesremn”t,ohpdehramihlieoerpihhdinelerreiZSnuydsneauetctzhigo”cveoolconcnugsseaitenuloesnn“g.vaiBteuliess“n2mb0e0ez1seoipwchhunirleedtne, Namensvettern wie Synechococcus elongatus PCC 7942 abgrenzte. Mit Hilfe von Elektroporation kann genetisches Material in die Zelle eingeschleust und ins Chromosom eingebaut werden (Nakamura et al 2002)
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