Engineering of Reversible Luminescent Probes for Real-time Intravital Imaging of Liver Injury and Repair

Abstract

Open AccessCCS Chemistry27 Feb 2021Engineering of Reversible Luminescent Probes for Real-time Intravital Imaging of Liver Injury and Repair Xiao Liu, Linhui He, Xiangyang Gong, Yue Yang, Dan Cheng, Juanjuan Peng, Lu Wang, Xiao-Bing Zhang and Lin Yuan Xiao Liu Google Scholar More articles by this author , Linhui He Google Scholar More articles by this author , Xiangyang Gong Google Scholar More articles by this author , Yue Yang Google Scholar More articles by this author , Dan Cheng Google Scholar More articles by this author , Juanjuan Peng Google Scholar More articles by this author , Lu Wang Google Scholar More articles by this author , Xiao-Bing Zhang Google Scholar More articles by this author and Lin Yuan Google Scholar More articles by this author https://doi.org/10.31635/ccschem.021.202100679 SectionsSupplemental MaterialAboutPDF ToolsAdd to favoritesTrack Citations ShareFacebookTwitterLinked InEmail As the primary organ for drug metabolism and detoxification, the liver is prone to be damaged and its functionality is severely impaired. The treatment of liver diseases is based on a clear understanding of the process underlying liver injury and repair. However, intravital real-time imaging of liver injury and repair is still limited due to the lack of in vivo reversible visualization methods. To this end, we proposed a rational design strategy for the development of reversible upconversion luminescence nanoprobe that allows real-time and in vivo imaging of liver injury and repair processes. As a proof of demonstration, we first developed a small molecule probe NB3 which can reversibly respond to related analytes of early liver injury (peroxynitrite, ONOO-) and liver repair (glutathione, GSH). The small molecule probe was then integrated with a core-shell upconversion nanoparticle to form a sophisticated nanoprobe. Compared to traditional small molecule probes, this nanoprobe exhibited higher selectivity to ONOO−, longer retention time in liver, and a wider response dynamic range to GSH after oxidized by ONOO−. The novel nanoprobe facilitated the successful monitoring and discrimination among the different degrees of liver injury and repair in a mouse model. Download figure Download PowerPoint Previous articleNext article FiguresReferencesRelatedDetails Issue AssignmentNot Yet AssignedSupporting Information Copyright & Permissions© 2021 Chinese Chemical Society Downloaded 137 times Loading ...