Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum.

Abstract

To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum. Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57%. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l. A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum.