Abstract
Random mutagenesis of Caldariomyces fumago was carried out to produce a chloroperoxidase (CPO) with enhanced activity in mixtures of aqueous buffers and organic cosolvents. A mutant CPO with a 3.4-fold increased activity in 40% aqueous tert-butyl alcohol was obtained.A CPO-surfactant conjugate, prepared by colyophilisation, mediated the oxidation of thioanisole to its (R)-sulfoxide by TBHP in water-saturated isooctane. Copolymerisation of CPO and a toluenediisocyanate prepolymer resulted in a stable preparation that mediated the oxidation of indole to 2-oxindole in a range of organic solvents. The highest yield was obtained in 1-octanol.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.