Engineering an activatable fluorescent probe for studying ONOO− in pyroptotic process

Abstract

Pyroptosis, a recently discovered form of programmed cell death, plays a pivotal role in oncological treatment. Howbeit, the mechanisms underlying pyroptosis in tumor treatment remain unclear. Previous research has demonstrated that the occurrence of pyroptosis generally accompanies a surge of reactive oxygen species (ROS) generation, with ONOO− being one of these ROS and closely linked to numerous diseases. Therefore, it is imperative to investigate the potential association between ONOO− and pyroptosis. Herein, a highly sensitive and rapidly responsive near-infrared (NIR) probe, Rd700-PN, is fabricated for exploring unrevealed relationships between ONOO− and pyroptosis. We successfully harness Rd700-PN to detect ONOO− fluctuation during cellular pyroptosis for the first time. Furthermore, the results demonstrate that Rd700-PN can scout the chemotherapeutic drug's induction ability of tumor pyroptosis in vivo. Notably, this study provides an excellent means to shed light on the correlation between ONOO− and pyroptosis and to screen antitumor drugs activating pyroptosis.