Engineered Tropoelastin and Elastin-Based Biomaterials

Abstract

Elastin is a key mammalian extracellular matrix protein that is critical to the elasticity, compliance, and resilience of a range of tissues including the vasculature, skin, and lung. In addition to providing mechanical integrity to tissues, elastin also has critical functions in the regulation of cell behavior and may help to modulate the coagulation cascade. The high insolubility of elastin has limited its use to researchers, while soluble derivatives of elastin including elastin peptides, digested elastins, and tropoelastin have much broader applications. Recombinantly produced tropoelastin, the soluble monomer of elastin, has been shown to exhibit many of the properties intrinsic to the mature biopolymer. As such, recombinant human tropoelastin provides a versatile building block for the manufacture of biomaterials with potential for diverse applications in elastic tissues. One of the major benefits of soluble elastins is that they can be engineered into a range of physical forms. As a dominant example, soluble elastins including tropoelastin can form hydrogels when they are chemically cross-linked. These self-organized constructs swell when transferred from a saline to aqueous environment and are highly elastic; these tunable responses are dependent on the types of cross-linker and elastin used. Soluble elastins can also be drawn into fine fibers using electrospinning. The morphology of these fibers can be altered by modifying spinning parameters that include delivery flow rate and the starting protein concentration. The resulting fibers then accumulate to form porous scaffolds, and can be wound around mandrils to create conduits for vascular applications. Electrospun scaffolds retain the elasticity and cell-interactive properties inherent in the tropoelastin precursor. Additionally, soluble elastins serve as versatile biomaterial coatings, enhancing cellular interactions and modulating the blood compatibility of polymer- and metal-based prostheses. Soluble elastins, and in particular tropoelastin, have highly favorable intrinsic physical and cell-interactive properties, warranting their adaption through incorporation into biomaterials and modification of implantable devices. The multiple choices of ways to produce elastin-based biomaterials mean that they are well suited to the tailoring of elastic biomaterials and hybrid constructs.