Abstract
As the number of manned space flights increase, studies on the effects of microgravity on the human body are becoming more important. Due to the high expense and complexity of sending samples into space, simulated microgravity platforms have become a popular way to study these effects on earth. In addition, simulated microgravity has recently drawn the attention of regenerative medicine by increasing cell differentiation capability. These platforms come with many advantages as well as limitations. A main limitation for usage of these platforms is the lack of high-throughput capability due to the use of large cell culture vessels. Therefore, there is a requirement for microvessels for microgravity platforms that limit waste and increase throughput. In this work, a microvessel for commercial cell culture plates was designed. Four 3D printable (polycarbonate (PC), polylactic acid (PLA) and resin) and castable (polydimethylsiloxane (PDMS)) materials were assessed for biocompatibility with adherent and suspension cell types. PDMS was found to be the most suitable material for microvessel fabrication, long-term cell viability and proliferation. It also allows for efficient gas exchange, has no effect on cell culture media pH and does not induce hypoxic conditions. Overall, the designed microvessel can be used on simulated microgravity platforms as a method for long-term high-throughput biomedical studies.
Highlights
In recent years the number of manned space flights has increased significantly
To further ensure that the PDMS microvessels allow for sufficient gas exchange in we demonstrated thegene microvessel not generate hypoxicfor conditions, cellAs culture conditions, that
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Summary
In recent years the number of manned space flights has increased significantly. Research has shown the importance of gravitational forces on the development and function of all living organisms and how microgravity conditions result in physiologically detrimental effects [1,2,3,4]. Due to this, simulated microgravity studies have become more widely used in recent years [6] Commercial platforms such as two- (2D), and three-dimensional (3D) clinostats, the rotating wall vessel (RWV), and the random positioning machine (RPM) are currently available and used to simulate the effects of microgravity on Earth [7]. These platforms have become recently used to increase cell differentiation capability for regenerative medicine [8,9,10,11]
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