Abstract
As alternatives to established carbohydrate‐detection reagents (antibodies and lectins), Lectenz® Bio is developing lectin‐like, enzyme‐derived proteins (known as Lectenz®), that target glycan sequences for which existing reagents either do not exist or are sub‐optimal. Lectenz® are engineered from catalytically inactivated glycan‐processing enzymes that have been optimized for high affinity towards specific glycan sequences. The conversion of such enzymes into affinity reagents is facilitated by computationally‐guided directed evolution. Lectenz® are being developed for a variety of glycan detection and enrichment applications including affinity chromatography, Western blot, and immunohistochemistry. Here we demonstrate the performance and specificity of two novel sialic acid recognizing Lectenz® engineered from a sialidase enzyme.Support or Funding InformationNIH R44 GM113351, R44 OD024964This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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