Abstract
Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E. coli strains for improved isoprene production, including K‐12 (DH5α, BW25113, W3110, MG1655, XL1‐Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5‐L fermentation, and scale‐up fermentation (300 L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.
Highlights
Isoprene is an important platform chemical that is widely used in the manufacturing of synthetic rubber along with various other applications such as the production of elastomers and adhesives; isoprene shows potential to be developed as a fuel additive for gasoline, diesel, or jet fuel [1,2,3]
Comparison of the isoprene production of E. coli strains in 5-L batch cultivation
We tested 11 E. coli strains as representatives of the wildtypes and their derivatives for comparison of volatile isoprene production
Summary
Isoprene is an important platform chemical that is widely used in the manufacturing of synthetic rubber along with various other applications such as the production of elastomers and adhesives; isoprene shows potential to be developed as a fuel additive for gasoline, diesel, or jet fuel [1,2,3]. Zurbriggen et al [12] reported the production of 320 mgÁLÀ1 isoprene from recombinant E. coli Rosetta (DE3) harboring the ispS gene from Pueraria montana along with an exogenous MVA pathway using sealed-flask cultivation. We previously obtained 12.7 gÁLÀ1 isoprene using E. coli DH5a with a two-vector system of Populus trichocapa ispS and the MVA pathway [15] Despite these numerous reports of enhanced isoprene production using several E. coli strains, all of these studies have focused on limited strains, including E. coli BL21, BL21 (DE3), BW25113 (DE3), Rosetta (DE3), and DH5a. Several E. coli strains are widely used as hosts for the production of recombinant proteins and metabolites, their performance and stability have not yet been directly compared We considered it necessary to investigate more E. coli strains for potential in enhancing isoprene production toward scale-up industrial application
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