Abstract
We present a new class of microspheres that have been designed with specific dielectric properties to enable their manipulation and identification in microsystems using dielectrophoretic methods. Self-assembled monolayers of alkanethiol and phospholipid were applied to gold-coated polystyrene microspheres. Microsphere populations having layers of different thicknesses were distinguishable and manipulatable by dielectrophoresis in a characteristic, frequency-dependent manner as predicted by theory. These proof of principle studies are the first step toward the development of an engineered bead-based dielectrophoretic microsystem for multiplexed molecular separation and analysis.
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