Engine oil from agricultural machinery as a source of PCDD/Fs and PCBs in free-range hens

Abstract

Free-range hens spend most of their lives outdoors, resulting in their heavy exposure to environmental pollutants such as polychlorinated dibenzo-p-dioxin, dibenzofurans (PCDD/Fs), dioxin-like polychlorinated biphenyls (DL-PCBs), and non-dioxin-like polychlorinated biphenyls (NDL-PCBs). We present a case of contamination of free-range eggs that is previously unreported in the literature. The aim of our study was a source investigation after finding a high level of PCDD/Fs in samples of eggs from one of the inspected farms. Samples of hens’ eggs, muscles, and livers and the feeds and soils were analyzed. The results showed that the soil samples taken from the paddock contained high concentrations of PCDD/Fs and DL-PCBs expressed as toxic equivalents (TEQ) (72.9 ± 18.2 pg WHO-TEQ g−1 dry mas (d.m.)) and a high concentration of NDL-PCBs (207 ± 46.9 ng g−1 d.m.). The investigation found that the cause of the soil contamination was oil leaking from the farm’s tractor engine. The oil contained very high concentrations of PCDD/F and DL-PCBs (1013 ± 253 pg WHO-TEQ g−1 oil) and 5644 ng g−1 of NDL-PCBs. The source of the contamination was confirmed by the similarity of the PCDD/F and PCB profiles in the hen eggs and the soil contaminated by engine oil. The dietary intake of toxins resulting from consumption of the eggs is provided. For children, the consumption of contaminated eggs would result in an intake of double the tolerable weekly intake (TWI), while for adults, it would be approx. 60–70% of TWI.