Enfeksiyöz ishallerde Campylobacter jejuni prevalansının çeşitli yöntemlerle araştırılması

Abstract

In our study, stool samples that were sent to the department of Microbiology for routine bacteriologic culture and parasitological examination were used in to estimate the prevalence of Campylobacter jejuni. Samples collected for 6 months. We depend on traditional methods such as culture for identifying the C. jejuni strains and molecular methods. Fresh diarrheal stool samples were streaked on Skirrow blood agar and incubated in a microaerophilic conditions at two different temperatures (35°C, 42°C) for 48 hours. Conventional methods were used for identification of C. jejuni. Antibiotic susceptibilities of the isolates were tested by disc diffusion method and the results were evaluated according to the EUCAST guidelines. All stool specimens were re-evaluated using (BD MAX™) Enteric Bacterial Panel system by real-time PCR method targeting tuf gen regions of C. jejuni/coli. From a total of 300 stool samples tested by both culture and molecular methods, only 3 C. jejuni strains were isolated by culture; however, using molecular method a total of 5 C. jejuni/coli positive were given.In conclusion, the culture for Campylobacter jejuni should be routinely included in our laboratories because of its importance as a bacterial cause of diarrhea in children. Also molecular methods are thought to be useful in the detection.