Abstract
The low -temperature fluorescence excitation analysis of different protochlorophyllide (PChlide) forms has been extended to the UV part of the spectrum . A new band at about 360 nm was detected in excitation spectra of active PChlide forms bound to isolated protochlorophyllide holochrome. This band is very similar to the absorbance of NADPH in this region and its intensity depends on the redox state of the surrounding medium. On illumination at low temperature the intensity of the band decreases considerably with out any corresponding changes in the redox state of “ free” NADPH in the surrounding medium. A new intermediate state exhibiting a mixed excitation spectrum between PChlide and chlorophyllide (Chlide) was detected in the course of PChlide photoconversion.
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