Energy production during anoxia and recovery in the adductor muscle of the file shell, Lima hians

Abstract

1. 1. During the initial period of anoxia (0–2.5 hr) 60% of the stored phosphagen of the adductor muscle was depleted, the levels of alanine increased and that of aspartate decreased. In the later period of anoxia (2.5–15 hr) there was a loss of 45% of the ATP content and the energy charge value dropped to 0.64. Lactate and octopine production were not observed. Formation of succinate took place during the whole anoxic period. 2. 2. During recovery the high energy status (phosphagen, ATP) was reached again after 1 hr as was the control level of succinate, whereas the concentration of alanine and aspartate were back to control values between 4 and 8 hr of recovery. 3. 3. The ATP consumption rate was greatly reduced in the later anoxic period. Glycolysis (first alanine, later succinate production) always contributed about 50%, arginine phosphate 47 and 27% and ATP 2 and 19% in the initial and later period, respectively.