Endurance training and LXR activation increased LXRα and ABCA1 transcripts in Wistar male rats

Abstract

Summary Purpose LXR, belongs to the ligands activated transcription factors, regulates the expression of genes in cholesterol homeostasis. The aim of the present study was to examine combinatory effects of selective LXR activator-T0901317 and training on gene expression profiles of LXRα and ABCA1. Material and methods To examine simultaneous effects of selective LXR activator-T0901317- and exercise on LXRα and ABCA1 genes expression and lipids profile, Wistar male rats were assigned to four groups as animal models: Control group (Ctrl), trained group received exercise (Exc) on a motor-driven treadmill at 28 meter/min for 60 min/day, 5 days/week for 8 weeks, group receiving LXR agonist T0901317 (10 mg kg−1 day−1) (Ago) for 1 week, group received both endurance training and LXR agonist T0901317 (Exc&Ago). Finally, rats were killed and blood was taken for lipids measurement. A portion of the liver was also excised for assessment of LXRα and ABCA1 transcripts. Results A significant increase in LXRα and ABCA1 transcripts was observed in Exc&Ago group when compared to other groups. However, there was not a synergistic response in plasma lipid and lipoprotein concentrations despite a significant increase in LXRα and ABCA1 mRNA levels, possibly due to a physiological negative feedback loop, the response to exercise could be negated by the effects of the agonist in plasma lipid and lipoprotein profiles.