Endothelium-independent relaxation to raloxifene in porcine coronary artery

Abstract

Although the vascular action of raloxifene has been studied in several vascular beds, the underlying mechanisms are still incompletely understood. The role of endothelium in raloxifene-induced vascular responses was controversial. The present study was designed to examine endothelium-independent effects of raloxifene in isolated porcine left circumflex coronary arteries. Arterial rings were suspended in organ baths and changes in isometric tension were measured. The large-conductance Ca 2+-activated K +(BK Ca) currents were recorded using a whole-cell patch-clamp technique. Treatment with raloxifene (1–10 μmol/l) reduced the contractions to 9,11-dideoxy-11α,9α-epoxy-methanoprostaglandin F 2α (U46619), serotonin (5-HT), endothelin-1 in normal Krebs solution and to CaCl 2 in a Ca 2+-free, high K +-containing solution. In endothelin-1-contracted rings, raloxifene (0.3 to 50 μmol/l) caused relaxations which were comparable in rings with and without endothelium. The raloxifene-induced relaxation was reduced by putative K + channel blockers, iberiotoxin and tetraethyl ammonium chloride (TEA +) in rings with and without endothelium, or by elevated extracellular K + ions (30 mmol/l K + and 60 mmol/l K +). 13-methyl-7-[9-(4,4,5,5,5-pentafluoropentylsulfinyl)nonyl]-7,8,9,11,12,13,14,15,16, 17-decahydro-6H-cyclopenta[a] phenanthrene-3,17-diol (ICI 182,780) did not affect raloxifene-induced relaxation. Raloxifene enhanced the outward BK Ca currents, which were sensitive to inhibition by iberiotoxin. In summary, the present study shows that raloxifene acutely relaxes porcine coronary arteries via an endothelium-independent mechanism without involving the ICI 182,780-sensitive estrogen receptors. Raloxifene mainly acts on the vascular smooth muscle cells to induce vasorelaxation by the inhibition of Ca 2+ channels and the activation of BK Ca channels. The former mechanism appears to play a more significant role.