Endothelin modulates the cardiovascular effects of clonidine in the rat

Abstract

Clonidine decreases mean arterial pressure (MAP) by acting as an α 2-adrenergic receptor (AR) agonist in the central nervous system; it also acts on peripheral α-ARs to produce vasoconstriction. Endothelin (ET) has been shown to modulate the action of ARs. The present study was conducted to determine the involvement of ET in cardiovascular effects of clonidine. Intravenous administration of clonidine (10, 30 and 90 μg kg −1) produced a dose-dependent decrease in MAP and heart rate (HR). Treatment with ET-1 (100, 300 and 900 ng kg −1) significantly attenuated clonidine (10 μg kg −1) induced fall in MAP and HR. Rats treated with ET-1 (900 ng kg −1) showed an increase in MAP and HR after clonidine administration compared to untreated rats, while ET A/B antagonist, TAK-044 (1 mg kg −1) and ET A antagonist, BMS-182874 (9 mg kg −1) potentiated the hypotensive effect of clonidine. ET B receptor agonist, IRL-1620 (5 μg kg −1) produced significant attenuation of clonidine induced fall in MAP and HR, while ET B receptor antagonist, BQ-788 (0.3 mg kg −1), potentiated the hypotensive effect of clonidine. Prazosin (0.1 mg kg −1) completely blocked ET-1 induced changes in cardiovascular effects of clonidine. Clonidine-induced contraction of rat abdominal aortic ring was potentiated by ET-1, which was completely blocked by prazosin. Clonidine produced an increase in ET A receptor expression in the brain and abdominal aorta while ET B receptors were not affected. It is concluded that ET enhances the responsiveness of vascular ARs to the constrictor effect of clonidine and ET antagonists potentiate the hypotensive effect of clonidine suggesting that a combination of ET antagonist with clonidine may be a useful option to treat hypertension.