Endothelin B Receptor is Involved in Endothelin-1 Induced Cell Response in Vascular Smooth Muscle Cells.

Abstract

Cardiovascular disease is an important problem in developed countries and the effective target of treatment should be further developed. ETB receptor is one of receptor of ET system, which may affect the function of vascular smooth muscle cell via NO released. In order to clarify the theory, we had cell culture with or without ET-1 treatment. Flow cytometry had been used to prove cell apoptosis and transwell assay had been used to detect the ability of cell migration. The expression of ET-1, ICAM-1, VCAM-1, MMP-9 and CRP were detected by using qRT-PCR and Western blot assay. After we had found that RES-701-1 one of ETB receptor antagonists can induced VSMC apoptosis and migration. Also RES-701-1 can down regulated ICAM-1, VCAM-1, MMP-9 and CRP while ET-1 was the opposite. In a conclusion ETB receptor is one of the role target to mediate vascular smooth muscle cell through the ET system and it may be a significant treating target in cardiovascular disease in the future.