Abstract
Conflicting evidence has been reported regarding the role of endothelin-1, a potent vasoconstrictor peptide, in stimulating extracellular calcium influx in rabbit vascular smooth muscle. The objective of this study was to elucidate the effects of endothelin-1 on transmembrane 45Ca 2+ influx and intracellular calcium mobilization in cultured rabbit aortic smooth muscle cells. In calcium containing buffer, endothelin-1 induced a concentration-dependent 45Ca 2+ efflux response over the range of 10 pM to 100 nM with an EC 50 of approximately 60 pM. Maximum endothelin-stimulated 45Ca 2+ efflux was not affected by the absence of extracellular calcium or the presence of 1 μM verapamil. Endothelin-1 did not induce transplasmalemmal 45Ca 2+ uptake at times up to 30 min. These findings suggest that an alteration in intracellular calcium handling, rather than extracellular calcium influx, is responsible for the endothelin-stimulated increase in intracellular calcium concentration in rabbit aortic smooth muscle cells.
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