Endothelial Dysfunction Improvement Mechanism By Hyperbaric Oxygen In Sprague Dawley By High-Cholesterol Diet

Abstract

The purpose of this study was to investigate the mechanism of hyperbaric oxygen could improve endothelial dysfunction. In the experimental method, Sprague Dawley strains divided 3 groups : the normal control for p1, high cholesterol diet for p2, and high cholesterol diet with hyperbaric oxygen of 2.4 ATA with 98% O2 for 3 sessions with the duration of 30 minutes / session, and air break for 5 minutes between each session for the period of 10 days consecutively for p3. On the last day of treatment, serum was taken for heme oxygenase-1 (HO-1), Sirtuin (SIRT1), endothelial Nitric Oxide Synthase (eNOS) and lipoprotein-associated phospholipase A2 (Lp-PLA2) by ELISA method. The new findings of this study are hyperbaric oxygen could improve endothelial dysfunction which occurs due to an atherogenic diet, through two pathways. The first, hyperbaric oxygen therapy increased heme oxygenase1 (HO1) (p = 0,000), sirtuin-1 (SIRT1) (p = 0.025), endothelial nitric oxide synthase (eNOS) (p = 0,000) and decreased levels of lipoprotein-associated phospholipase A2 (Lp-PLA2) (p = 0,000). The second hyperbaric oxygen administration enhances sirtuin1 (SIRT1) (p=0,000) directly, endothelial nitric oxide synthase (eNOS) (p = 0,000) and decreased levels of lipoprotein-associated phospholipase A2 (Lp-PLA2) (p = 0,000).