Endothelial cell‐mediated formation of high density lipoprotein‐like particles

Abstract

Passage of plasma triglyceride‐rich lipoproteins (TRLs) and low‐density lipoproteins (LDLs) through the monolayer endothelium occurs in normal and atherosclerotic arteries. The current report studied the effect of transendothelial transport on the density distribution of TRL‐ and LDL‐associated cholesterol and apolipoproteins (apo). Our data indicated that the 3H‐cholesterol and 125I‐apoE in the TRLs and LDLs that were not incubated with the mouse aortic endothelial cell (MAEC) monolayer distributed primarily in the low density (LD) fractions (d: ≤1.063). In contrast, a substantial portion of the 3H‐cholesterol and 125I‐apoE that had passed through the MAEC monolayer were allotted into the high density (HD) (d: ≥1.063) fractions. ApoB protein was detectable only in the LD fractions before or after TRLs or LDLs were incubated with the MAEC monolayer. Inhibition of caveolae‐dependent endocytosis reduced the transendothelial transport of TRLs and LDLs and its associated apoE‐carrying HD particle formation. These suggest that TRLs and LDLs pass through the MAEC monolayer in the forms of apoB‐carrying LD particles and apoE‐carrying HD particles.Support or Funding InformationNIH SC1HL101431This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.