Abstract
Oxidative stress can lead to significant interactions between endothelium and leukocyte, which is implicated in the pathogenesis of many diseases. In this study, we analyzed the interactions of human umbilical vein endothelial cell (HUVEC) and human acute monocytes leukemia cell (THP‐1) line under the treatment of high glucose and hydrogen peroxide (H2O2). Cells were treated with high glucose (25 mM) and/or H2O2 (10, 50, 100 μM) for 24 hours. ROS levels and cell adhesion were measured using dihydroethidium fluorescence and calcein‐AM fluorescence via microscopy and micro‐plate reader. The ROS levels increased with the increase concentration of high glucose and H2O2 treated HUVEC. The adhesion of normal glucose treated THP‐1 cells on HUVEC increased with the increase concentration of high glucose and H2O2 treated HUVEC. High glucose treated THP‐1 cells can increase the adhesion of THP‐1 and HUVEC compared with normal glucose treated THP‐1. These results show us that both high glucose and H2O2 can increase the ROS levels and the adhesion of THP‐1 and HUVEC cells. Supported by NIH grant # R01 HL084337.
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