Endosymbiont Communities in Pachyseris speciosa Highlight Geographical and Methodological Variations

Sudhanshi S Jain,Lutfi Afiq-Rosli,Bar Feldman,Benjamin J Wainwright,Oren Levy,Danwei Huang,Ismael Kunning,Ralph R Mana

doi:10.3389/fmars.2021.759744

Abstract

Reef-building corals live in symbiosis with the phototrophic dinoflagellate family Symbiodiniaceae, which comprises diverse genera such as Cladocopium and Durusdinium. Pachyseris speciosa, a widely distributed Indo-Pacific coral found in a variety of reef habitats, is known to be associated with these two Symbiodiniaceae genera, but little is known about the biogeographic variability of the endosymbiont communities across the region. In this study, the diversity and dominance patterns of Symbiodiniaceae at the western and eastern areas of the Central Indo-Pacific region were examined. We sampled Pachyseris speciosa colonies at seven and six sites in Singapore and Papua New Guinea, respectively, and genotyped their endosymbionts based on the internal transcribed spacer (ITS) markers using two distinct methods, quantitative polymerase chain reaction (qPCR) and high-throughput sequencing (HTS). Results showed 92% of all colonies in Singapore exhibiting Cladocopium dominance. There was a higher abundance of Durusdinium compared to Cladocopium in certain colonies from one site, Pulau Hantu (mean Durusdinium abundance of 90%, compared to 0–14% among all other sites). In contrast, variation in the endosymbiont communities was generally higher among sites in Papua New Guinea. Cladocopium expectedly dominated most colonies (75%), although colonies from Kimbe Bay (85%) and Kavieng (65%) showed Durusdinium dominance. Between localities, relative genus abundances based on qPCR were not significantly different, but HTS showed that the ratio of Durusdinium over Cladocopium was significantly higher in Papua New Guinea corals. Notably, 6% of colonies from Singapore and 15% from Papua New Guinea showed endosymbiont dominance patterns that were inconsistent between the two methods, underscoring the need for further validation of symbiotic algal quantification based on HTS. The richness of ITS2 type profiles was clearly lower among colonies from the impacted and turbid reefs of Singapore compared to the less urbanized reefs of Papua New Guinea. These coral intraspecific variations of Symbiodiniaceae communities within and among localities suggest that local conditions are important drivers of endosymbiosis and may ultimately influence corals’ resilience against global stressors such as ocean warming.

Highlights

Reef-building stony corals (Cnidaria: Anthozoa: Scleractinia) are associated with the dinoflagellate alga Symbiodiniaceae, forming an ecologically critical symbiotic relationship (Falkowski et al, 1984; Stanley and Fautin, 2001; Baker, 2003)
In accordance with the SymPortal framework (Hume et al, 2019), we report on the internal transcribed spacer 2 (ITS2) type profiles of P. speciosa that account for intragenomic variation and are representative of putative Symbiodiniaceae species
The quantitative polymerase chain reaction (qPCR) quantification of the relative abundances of Durusdinium vs. Cladocopium internal transcribed spacer 1 (ITS1) in the 146 colonies sampled across Singapore showed limited variation among the seven sites examined

Summary

Introduction

Reef-building stony corals (Cnidaria: Anthozoa: Scleractinia) are associated with the dinoflagellate alga Symbiodiniaceae, forming an ecologically critical symbiotic relationship (Falkowski et al, 1984; Stanley and Fautin, 2001; Baker, 2003). In hospite Symbiodiniaceae cells are provided with a lightenriched environment and the by-products of host metabolism (Banin et al, 2003). These extremely diverse microalgae are currently recognized as several phylogenetically distinct genera (LaJeunesse et al, 2018; Boilard et al, 2020). Reef-building stony corals typically host multiple Symbiodiniaceae species and are most commonly associated with four genera (Symbiodinium, Breviolum, Cladocopium, and Durusdinium) (Rowan and Knowlton, 1995; Baker, 2003; Little et al, 2004; Stat et al, 2008b). The endosymbiont community may grant varying degrees of fitness to the coral host depending on the holobiont’s capability to tolerate environmental stressors and bleaching (Baker, 2001; Stat et al, 2011)

Methods

Results

Conclusion