Abstract
The aim of this study was to explore the electronic structure of the radical cation of the primary electron donor in the PSII reaction centre, P680, using ENDOR spectroscopy. This technique has been used to elucidate the electronic structure of the oxidised primary donors in purple bacteria (1) and P700 (2,3). However, there has only been one report of similar ENDOR studies on P680+· (2). Study of P680+· is hampered by the fact that its very high redox potential results in oxidation of secondary electron donors (4–6). Here we have compared untreated PSII RC with samples in which secondary donors were inactivated.
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