Endoplazmik retikulum stres belirteçlerinden ATF5 ve fosforile eIF2α düzeylerinin nöroblastoma hücrelerinde kainik asit muamelesi sonrası incelenmesi

Abstract

Purpose: The aim of this study was to investigate the relationship between kainic acid induced excitotoxicity and endoplasmic resticulum (ER) stress by analyzing two major ER stress markers such as ATF5 and phosphorylated eIF2 in neuroblastoma cells. Materials and Methods: Neuroblastoma cells were treated with 1 mM kainic acid for 24 hours. ATP measurement was performed in kainic acid-treated and vehicle-treated neuroblastoma cells via ATP bioluminescence assay. Total protein was isolated from kainic acid-treated and control cells. Via western blotting, the expression levels of ATF5 and phosphorylated eIF2α were analyzed.Results: We showed for the first time that as a result of kainic acid treatment in neuroblastoma cells, the protein expression levels of ER stress markers ATF5 and phosphorylated eIF2 did not display any change when compared to control cells. We also showed that ATP levels were decreased in kainic acid-treated cells.Conclusion: This study may show that the level of stress that kainic acid causes at 1 mM for 24 hours in neuroblastoma cells was not adequate to lead to ER stress which is measurable by ATF5 and phosphorylated eIF2. Either an increased level of treatment of kainic acid via increased duration or concentration is necessary or different markers should be tried. The investigation of the ER stress pathways in the excitotoxicity-related brain diseases will pave the way for new therapies based on ER stress and combat more than one disease simultaneously.