ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS

GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA,SILVIA NIETSCHE,DEIVISSON FERREIRA DA SILVA,MARLON CRISTIAN TOLEDO PEREIRA,ADELICA APARECIDA XAVIER

doi:10.1590/0100-29452017324

Abstract

ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid producers have provided significant increases for length, pseudostem diameter, fresh masses and dry masses in ‘Prata Anã’ micropropagated banana seedlings.

Highlights

The micropropagated banana seedlings originate from small shoot apices, called explants, that after the aseptic phase are grown under laboratory aseptic conditions in artificial culture media and controlled conditions of temperature, photoperiod and luminosity (NGOMUO et al 2014)
The endophytic bacteria used in the present study were isolated and identified by Souza et al (2013) and the evaluation of their in vitro biotechnological potential was carried out by Andrade et al (2014)
All components of fresh mass evaluated were affected by the inoculation of the endophytic bacteria described as biological fixatives of N

Summary

Introduction

The micropropagated banana seedlings originate from small shoot apices, called explants, that after the aseptic phase are grown under laboratory aseptic conditions in artificial culture media and controlled conditions of temperature, photoperiod and luminosity (NGOMUO et al 2014). The micropropagation technique provides a clearing of the pathogenic microbiota as well as of the natural associated with the plantlets. This condition is generally extended when plants are transferred to soils or substrates in the initial stages of acclimatization (AHMED et al, 2014). The absence of a diversified microbiota associated with the rhizosphere of plants can reduce growth and vigor and increase the mortality of seedlings in the field. The endophytic organisms find in the plant a habitat with nutrients and less competition with other microorganisms (PEIXOTO NETO et al, 2004)

Objectives

Methods

Results