Endophytic bacteria mediated anti-autophagy and induced catalase, β-1,3-glucanases gene in paddy after infection with pathogen Pyricularia grisea

Abstract

Endophytic bacteria inhabiting in plant root and facilitate the plant growth and health. Among 28 isolates, two isolates Pseudomonas aeruginosa and Pseudomanas pseudoalcaligenes were selected to evaluate their ability as a biological agent against fungal pathogen Pyricularia grisea responsible for fungal blast in paddy. The paddy plants were inoculated with these isolates during the initial stage of growth and latter infected with fungal pathogen under greenhouse condition. The observation showed that, the two endophytic isolates were able to activate phenolics and flavonoids production and induction of PR proteins like enzymes β-1,3-glucanase and catalase in paddy even in absence of pathogen and when such plant infected by fungal pathogen like Pyricularia grisea, it showed more resistant against it. Production of such enzymes due to endophytic bacteria resulted in reduction of its autophagy dependent cell death as reduced red fluoroscence in bacterized plant cell, observed under fluorescence microscope and also induction of β-1,3-glucanase and catalase gene in paddy. Uses of such beneficial bacteria in diverse agro-ecological system can be broadly use to develop pre-formed defence activation to develop resistance against broad range of pathogen in important crops.