Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa Wallich) as Antibacterial Agent

Maria Yasinta Moi,Sri Pujiyanto,Endang Kusdiyantini

doi:10.15294/biosaintifika.v10i3.15361

Maria Yasinta Moi, Sri Pujiyanto + Show 1 more

Open Access

https://doi.org/10.15294/biosaintifika.v10i3.15361

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Abstract

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.

Highlights

The use of plants as a source of herbal medicine increases along the terracing of infectioncause bacteria’s resistance against some various antibiotic drugs
Six isolates of endophytic bacteria were isolated from Faloak Plant Seed from Kupang City, East Nusa Tenggara Province
The isolates were tested for antibacterial activity against testing bacteria Staphylococcus aureus and Escherichia coli

Summary

Introduction

The use of plants as a source of herbal medicine increases along the terracing of infectioncause bacteria’s resistance against some various antibiotic drugs. The use of the herbs which are directly taken off from their parent plant, is feared could reduce the population of that plant itself. An alternative is clearly necessary to maintain the plant’s sustainability, its population can live longer and can be far from extinction. Endophytic microbes live in the plant tissue without affecting its host plants. The endophytic microbes which is isolated from a herbal plant can produce the identical secondary metabolites like the host plants’s, even its amount will be higher when the fermentation process is applied. Compared to the method of the active compounds bacteria-extraction, it needs more complicated process and requires such a long time to gain the active compounds directly from the plant (Purwanto, 2014)

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