Abstract
Soybean ( Glycine max (L.) Merrill) is a universal important plant. Its production has decreased each year. A major problems is a pest known as Aphis glycine Matsumura (Hemiptera: Aphididae) which caused the decline of yield up to 40%. Thus, there is a need to control A. glycine . Recent studies showed B . bassiana has a potency as endophyte in Vicia faba (Fabaceae) plant tissue. It could harm A. gossypii and causes death up to 57,7%. Therefore, the aim of this research is to control infestation of A. glycine by using B. bassiana . This research used Randomized Block Design with soybean plant Anjasmoro variety as samples. The initiation of B. bassiana was conducted by using seed-soaking and soil-wetting methods. The results showed that fungus B. bassiana was able to colonize soybean plants with a percentage of colonization reaching 6.67%. Colonization was only found in the stems of soybean plants in the seed immersion treatment with the susceptibility of B. bassiana fungus in density of 108 conidia / mL for 2 hours, colonization on the stem itself by 20%. Fungus density results from evaluation of 1.6 x 105 conidia / mL and conidial viability reached 87.4%. There were no deaths and changes in the population of A. glycine aphids. Direct pathogenicity testing has a significant effect on the treatment between observations of the second day. Initiation of B. bassiana fungi did not significantly affect plant height and number of leaf branches. This results conclude that B. bassiana may function as endophyte in soybean plants. However, its function in reduction of greenfly still need further research Keywords: Biological control, Environmental pest management, Greenfly
Highlights
INTRODUCTION1 Soybean (Glycine max (L.) Merrill) is a plant that has important value as a source of vegetable protein with an average production of the last two years reaching 968,982 tons and experiencing an increase [1]
The results showed that fungus B. bassiana was able to colonize soybean plants with a percentage of colonization reaching 6.67%
Colonization was only found in the stems of soybean plants in the seed immersion treatment with the susceptibility of B. bassiana fungus in density of 108 conidia / mL for 2 hours, colonization on the stem itself by 20%
Summary
(RCBD) with 4 treatments and 5 replications, so as to obtain 20 experimental units. The soybean plants used are Anjasmoro varieties. Research implementation includes fungus initiation, direct pathogenicity testing, endophytic pathogenicity testing, and evaluation. The implementation of the research included fungal initiation, direct pathogenicity testing, endophytic pathogenicity testing, and evaluation. The initiation of fungi was carried out on soybean plants aged 5 days using the seed immersion method and soil wetting with a density of B. bassiana 1 x 108 conidia / mL. Direct pathogenicity testing was carried out with density levels of 105, 106, 107, and 108 conidia / mL and observed for 7 days. Endophytic pathogenicity testing was carried out by infesting 20 crop aphids at age 20 dai and observing the growth aspects of plant height and number of leaf branches. Evaluation was carried out three times on soybean plants aged 5, 20 and 30 dai, respectively. This evaluation is carried out destructively and isolates samples of each part of the plant on PDA media which has been added with antibiotics
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