Abstract
Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.
Highlights
Endometriosis is an abundant gynecological pathology of poorly understood pathogenesis affecting 10% of women[1]
Features of different tissues are identified by conventional lipidomic approach including lipid extraction and following analysis by hydrophilic interaction liquid chromatography with electrospray ionization mass spectrometry (HILIC-LC/ESI-MS)[16, 17]
The following settings are varied until obtaining stable total ion current (TIC) in both positive and negative ion modes: distance from tissue tip to the mass spectrometer inlet is varied in the range 5–50 mm; applied potential in the range 2–5 kV, extracting solvent flow rate in the range 5–50 μL/min
Summary
Endometriosis is an abundant gynecological pathology of poorly understood pathogenesis affecting 10% of women[1]. Features of different tissues are identified by conventional lipidomic approach including lipid extraction and following analysis by hydrophilic interaction liquid chromatography with electrospray ionization mass spectrometry (HILIC-LC/ESI-MS)[16, 17]. The following settings are varied until obtaining stable TIC in both positive and negative ion modes: distance from tissue tip to the mass spectrometer inlet is varied in the range 5–50 mm; applied potential in the range 2–5 kV, extracting solvent flow rate in the range 5–50 μL/min.
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