Endometrial receptivity evaluation in IVF cycles

Abstract

Endometrial receptivity formation in in vitro fertilization (IVF) cycles was assessed both on molecular level: leukemia-inhibitory factor (LIF), transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), granulocyte macrophage colony stimulating growth factor (GM-CSF) together with hemodynamic characteristics of uterine (Ua) arteries.Aim: Aim of study was to working out an endometrial receptivity evaluation-based prognostic model for IVF efficacy in examined patients.Materials and methods: A total of 97 infertility patients in IVF cycles enrolled in the study. Тwo groups were formed, retrospectively. Group I included 50 women with ultrasound-confirmed pregnancy (51.55%), while the second one embraced 47 women with failed pregnancy. Within the pre-supposed “window of implantation” (WOI) in the preceding IVF cycle, endometrial biopsy was done, succeeded by immunohistochemical assessment of LIF, VEGF and TGF-β1 expression in endometrial samples. The VEGF, GM-CSF and TGF-β1 content in the cervical mucus (CM) was measured by multiplex analysis on transvaginal puncture (TVP) and embryo transfer (ET) day. Hemodynamic characteristics (systolic/diastolic rate, S/D), resistance index (RI) and pulsation index (PI) of Ua arteries on the 2nd–3rd day of menstrual cycle, ovulation triggering (OT) and ET day were assessed.Results: Based on logistic regression analysis, it was found that LIF expression in endometrial epithelium obtained on WOI period of the preceding IVF cycle; VEGF content in the CM on the TVP day, S/D rate and RI of spiral arteries on the OT day are of significant importance in endometrial receptivity formation. Built math model allows high accuracy (85.6%) prediction for pregnancy achieved through IVF by means of applying the suggested parameters.