Abstract
The role of endo-β-mannanase (EBM) during lettuce seed germination at 35°C and the influencele of ethylene in EBM regulation were investigated. Seeds of ‘Dark Green Boston’ (DGB) and ‘Everglades’ (EVE) were germinated in water, 1-aminocyclopropane-1-carboxylic acid (ACC), or aminoethoxyvinylglycine (AVG). Seeds were also primed in polyethylene glycol (PEG), or PEG + ACC, PEG + AVG. Untreated seeds germinated 100% at 20°C. At 35°C, EVE germinated 100%, whereas DGB germinated only 4%. Seed priming or adding ACC during incubation increased germination at 35°C. AVG inhibited seed germination of DGB at 35°C. Higher enzyme activity was observed in EVE compared with DGB seeds. Providing ACC either during priming or during germination increased EBM activity, whereas AVG decreased activity. Higher ethylene production was detected in EVE than DGB during germination at 35°C. The results suggest that ethylene overcomes the inhibitory effect of high temperature in thermosensitive lettuce seeds by weakening of endosperm due to increased EBM activity.
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