Abstract
Steroid [ 35S]sulfates can be formed from endogenous acceptors upon incubation of certain tissue extracts with [ 35S] sulfate, or adenosine-3′-phosphate-5′-phospho[ 35S] sulfate of high specific activity. These radioactive components may possess abnormal paper Chromatographic properties and addition of steroid to the incubations can specifically increase the radioactivity therein without giving rise to a product in the expected position for authentic non-labeled steroid sulfate. Addition of the latter as carrier prior to chromatography overcomes this R F variation, suggesting that the abnormal Chromatographic properties are caused by the employment of ‘sub-chemical’ concentrations. The steroid sulfate formed from endogenous steroid in the case of embryonic chick cartilage extracts has been identified as estrone sulfate.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
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