Abstract

Abstract Acidic ethyl ether-soluble growth substances extracted from sour cherry (Prunus cerasus L. cv. Montmorency) seeds were subjected to repeated paper chromatography. A growth promoting substance was isolated, active in both the Avena first internode and Avena curvature bioassays, with chromatographic and chromogenic properties similar to those of indole-3-acetic acid (IAA). This tentative identification was strengthened by gas-liquid co-chromatography of the trifluoroacetyl derivatives of authentic IAA and the endogenous growth promoter. Conclusive confirmation that the growth promoting substance is IAA was provided by combined gas chromatography-mass spectrometry.

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