Abstract
Summary Root formation in Sequoiadendron giganteum cuttings from in vitro culture was accompagnied by at first a rise and then a fall in soluble peroxidase (EC 1.11.1.7) activity in the explant. A change in the opposite direction (a fall followed by a rise) was shown for free IAA as measured by a solid-phase enzyme immunoassay using specific anti-hormone antibodies. The levels of abscisic acid, zeatin and zeatin riboside estimated by similar immunological techniques increased up to the sixth day on the rooting medium, passed through a minimum on day 13 and then reincreased slightly. These variations were compared to those described in the literature. A scheme is presented showing the possible events connecting rooting and variations of endogenous plant hormones and isoperoxidases during rooting process of Sequoia.
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