Endogenous levels of abscisic acid, indole‐3‐acetic acid, zeatin and zeatin‐riboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes

Abstract

Indole acetic acid (IAA). abscisic acid (ABA), and zeatin plus zeatin riboside (Z + ZR) were determined daily in cuttings of Lycopersicon esculentum Mill. cv. Craigella (C) and the Craigella Lateral Suppressor (CLS) mutant during the first 5 days of the root‐forming process. A solid‐phase enzyme immunoassay using specific anti‐hormone antibodies was used following a one‐step HPLC purification procedure. The hormone measurements were made in cuttings divided into 4 parts. The main variations occurred in the terminal bud and in the basal part of the hypocotyl of the two tomato varieties, i.e. significant IAA and ABA increases during the first 2 days followed by a more or less fast return to the initial values at day 4 or 5. This is probably due to the ablation of the root system. Z + ZR levels dramatically decreased in the basal part of the Craigella hypocotyl 1 day after cutting. Contrary to CLS, C hypocotyls recovered the initial high levels when roots regenerated (day 5). This is probably linked to the greater ability of roots to produce cytokinins in C plants than in CLS ones. The first step of root formation (reactivation of the pericyclic cells and formation of root primordia) corresponded to a high IAA/Z + ZR ratio in the root‐forming tissue, whereas the second step (elongation of young roots) was characterized by a low ratio with low levels of hormones.