Endogenous Intoxication and Saliva Lipid Peroxidation in Patients with Lung Cancer.

Lyudmila Bel’skaya,Gilbert Massard,Victor Kosenok

doi:10.3390/diagnostics6040039

Abstract

This research was aimed at a search for regularities in changes to parameters of endogenous intoxication and saliva lipid peroxidation in patients with lung cancer, non-malignant lung diseases, and apparently healthy people. All patients went through saliva sampling at an amount of 1 mL. A concentration of malondialdehyde (MDA) was measured according to a reaction with thiobarbituric acid, and a level of middle molecules (MM) was measured with UV spectroscopy at 254 and 280 nm, while the content of lipid peroxidation products was measured according to a degree of heptane extract light absorption at wavelengths of 220, 232, 278, and 400 nm. It has been revealed that in the context of lung cancer, the level of diene conjugates decreases, increasing the level of triene conjugates, Schiff’s bases, and MM. As a tumor grows, there is a decrease in the level of lipid peroxidation primary products and an increase in endotoxemia phenomena. The process is more apparent when going from local to locally advanced disease states. The nature of the MDA change is nonlinearly associated with tumor progression. The findings might be used to optimize traditional aids of diagnostics, in disease state forecasting, in treatment monitoring, etc.

Highlights

The elaboration of aids in diagnostics and forecasting of lung cancer course, a top oncologic disease [1,2,3,4], is still a priority
An excessive formation of reactive oxygen species, which, besides their evident mutagenic effect, initiate lipid peroxidation processes in biological membranes, is a crucial factor involved in the mechanisms of carcinogenesis
It is associated with reactive oxygen species that damage mitochondrial DNA, which leads to bioenergetic crisis and a release of apoptotic protease activating factors into the cytoplasm [5,6]

Summary

Introduction

The elaboration of aids in diagnostics and forecasting of lung cancer course, a top oncologic disease [1,2,3,4], is still a priority. The oxidative stress that occurs in a body is considered a key process in changes to programs of cell differentiation, proliferation, and apoptosis. It is associated with reactive oxygen species that damage mitochondrial DNA, which leads to bioenergetic crisis and a release of apoptotic protease activating factors into the cytoplasm [5,6]. Free radicals are involved in all stages of tumor development, starting from the moment of malignant transformation in cells They contribute to tumor growth and its invasive and metastatic potential, and trigger a cascade of reactions that lead to irreversible consequences in the cell [7]. Endogenous intoxication occurs accompanied by the increased level of middle molecules (MM)

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