Abstract

Endogenous levels of indolyl-3-acetic acid (IAA) were monitored during the growth of plant tissue callus cultures using the pyione fluorimetric assay. Tissue IAA concentrations in sterile auxin-autotrophic cultures derived from crown gall tumors rose after subculture to a maximum and subsequently declined. In auxin-dependent cultures derived from healthy plant tissues and maintained on 2,4-dichlorophenoxyacetic acid-containing medium endogenous IAA levels were lower than in corresponding crown gall cultures and showed no consistent pattern of change during growth after subculture. These differences are discussed in relation to hormonal control of plant tissue growth.

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