Abstract
Focal adhesions (FAs) are key nodes of force transmission between the cell and the extracellular matrix (ECM) in many cell types. One specific FA protein, vinculin, is essential for myosin contraction-dependent adhesion strength. As FAs are mechanosensitive structures, it is important to quantitate the specific amount of force sensed at these adhesion complexes and the relative distribution within cells that may be involved in mechano-regulation of downstream cellular processes. A recently developed tool to study FA tension is a genetically coded FRET (Förster resonant energy transfer) tension biosensor, where two fluorophores are separated by a linker of known stiffness.
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