Endocytosis and transcytosis by the capsule cell of vertebrate muscle spindles

Abstract

The vertebrate muscle spindle has been observed to be ionically and biochemically isolated from the surrouding muscle fibers by the spindle capsule. We have explored the possibility that the capsular cells are endocytically active and can transport both small molecules and macromolecules into the capsular space. Transcytosis (the endocytic transport of extracellular substances across a cell) through the capsule layer was examined with muscle spindles of snake, rat, and cat using fluorescent markers for fluoresence microscopy and horseradish peroxidase (HRP) and ferritin for electron microscopic examination. The fluorescent markers were actively taken up by capsule cells, making it easy to locate the spindle capsular region of spindles among extrafusal fibers by their strong fluorescence. Ferritin and HRP were used to identify the pathway of transcytosis by electron microscopy. These markers were found in endocytic vesicles of capsule cells, in the narrow space between capsule layers and in the capsular space, indicating that the marker was transferred to the capsular space by the pinocytic activity of capsule cells. Scattered cells in the capsule of cat muscle spindles appeared to take up fluorescein isothiocyanate (FITC)-coupled β-glucuronidase by a receptor-mediated process. The uptake was sensitive to temperature and [Ca 2+], and specifically inhibited by yeast mannan. By electron microscopy with dilute HRP (10 μg/ml) this specific uptake was by isolated cells in the interlamellar space. The functional significance of the above findings is discussed.