Abstract
The present study examines the mode of action of eyestalk factors, in particular crustacean hyperglycaemic hormone (CHH), on known target tissues (muscle and hepatopancreas) in two decapod crustacean species,Nephrops norvegicusandCarcinus maenas.The possibility that receptors to CHH are coupled to an elevation of cytosolic Ca2+derived from intracellular stores via activation of the phosphatidylinositol pathway was investigated. Using an inositol 1,4,5-trisphosphate (InsP3)-specific competitive binding assay, measurable levels of InsP3were detected in hepatopancreas and muscle in bothN. norvegicusandC. maenas.Incubation of these tissues in the presence of sinus gland extracts (two sinus gland equivalents) consistently increased InsP3. In addition,CarcinusCHH (10 nM) increased InsP3levels inCarcinushepatopancreas and muscle. Incubation of hepatopancreas from both species in 10 mMlithium saline increased basal levels of InsP3, suggesting a significant turnover of phosphoinositides in this tissue.
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