Abstract

SummaryA method has been described for testing the endocrine function of ovarian tissue after growth in culture at 37°C or storage at 10°C. Such tissue, when transplanted into the anterior chamber of the eyes of castrate female rats, after a latent period of from 8 to 24 days, produced typical estrous type of vaginal smears which compared favorably with the effects produced by transplants made directly after the removal of the tissue from the donor animal. Ovarian tissue frozen quickly in liquid air failed to produce cornification of the vaginal epithelium.

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